Reporter

Part:BBa_J44012:Design

Designed by: Todd Eckdahl   Group: iGEM06_Missouri   (2006-10-26)


HixC-pBAD-HixC-RBS-TetF-HixC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 159
    Illegal NheI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 99
    Illegal BamHI site found at 517
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
    Illegal NgoMIV site found at 911
    Illegal NgoMIV site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Trascription readthrough into part appears to have been blocked by the introduction of double forward terminators before and after the cloning site.


Source

Cloned from BBa_J44010

References